General Cell Based Assays
Eurofins Discoverx PathHunter eXpress TrkC Functional Assay, 2-Plate Kit. (93-0464E3CP16M)
The PathHunter eXpress TrkC Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkC dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkC and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human IL-17A/F ASY
The Meso Scale Discovery U-PLEX Human IL-17A/F Assay Kit is a multiplex immunoassay that measures human interleukin 17A and interleukin 17F in cell culture supernatants serum plasma and other biological samples This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies Read Buffer Diluent 2 Calibrator Blend and Wash Buffer It can detect IL-17A in a range of 0 06-1500 pg/mL and IL-17F in a range of 0 10-1500 pg/mL
Meso Scale Discovery U-PLEX Human IL-1 ASY
The Meso Scale Discovery U-PLEX Human IL-1alpha Assay Kit is a multiplex immunoassay that measures IL-1alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay can quantify IL-1alpha concentrations between 0 06 pg/mL to 10 000 pg/mL It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and an MSD read buffer The total assay time is 3 5 hours
Abcam Thioredoxin Reductase Assay Kit (Colorimetric).
Thioredoxin Reductase Assay Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Rhod-4 Calcium Assay kit - Medium Removal No Wash.
Rhod-4 Calcium Assay kit - Medium Removal No Wash.
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences PROTEOSTAT Aggresome detection kit (1x100tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT. . Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent . Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Hach Company SC4500 Ctrl Prog Prof DP 1 mA
SC4500 Controller-Prognosys-Profibus DP-1 mA Input-100-240 VAC-without power cordIncludes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
Cell Biolabs Inc OxiSelect™ Superoxide Dismutase Activity Assay Kit, 100 assays
Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, are constantly produced during metabolic processes in all living species. Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. The OxiSelect™ Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. The assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, SOD standards and unknown protein samples.
Abcam MnTBAP chloride, Supero x ide dismutase (SOD) mimetic, 10MG
MW 879.1 Da. Cell-permeable SOD mimetic. Potent peroxynitrite scavenger. Does not scavenge NO. Shows anti-inflammatory effects and reduces oxidative injury in vivo. Reduces ROS and shows a neuroprotective effect in spinal cord injury.
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery RPLEX HUM Influenza A NP ASY
The R-PLEX Human Influenza A NP Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that simultaneously detects influenza A nucleoprotein and differentiates between H1N1 and H3N2 subtypes in human nasopharyngeal swab specimens This assay utilizes MSD s electrochemiluminescent detection technology and provides results in under 5 hours It is intended for research use only and not for use in diagnostic procedures The kit includes a 96-well plate coated with capture antibodies detection antibodies read buffer wash buffer blocker solutions calibrators and controls
Eurofins Discoverx AssayComplete Freezing Reagent F1, 2 x 50. (92-5101FR)
AssayComplete Freezing Reagent F1 is an optimized freezing reagent for cryopreservation of Eurofins DiscoverX cell lines. This reagent ensures optimal cell viability and assay performance upon thawing. This product is provided as 2 bottles of 50 mL reagent each. Please refer to cell line specific datasheet to determine the recommended freezing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress FPRL1 Activated GPCR Internalization Assay, 10pl Kit. (93-0666E3CP0L)
The PathHunter FPRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of FPRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged FPRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting FPRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyChrom™ Superoxide Dismutase Assay Kit. For quantitative determination of superoxide dismutase (SOD) enzyme activity.
For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects. Key Features: Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD440nm. Samples: Blood, cell, tissue etc. Species: All. Procedure: Assay takes 1 hr. Kit size: 100 tests. Detection limit: 0.05 U/mL.
Bioassay Systems PiBlue™ Phosphate Assay Kit. For sensitive and high-throughput phosphate determination.
For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure". Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.